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Before evaluation, participants washed their faces using face cleanser, soap, and water

Hydrogen peroxide produced by neutrophils is used mainly by MPO which catalyzes oxidation reactions with the involvement of chlorine ions to produce hypochlorous acid (HOCl), hydroxyl radicals (OH ), singlet oxygen (O 2 1 ), and ozone (O 3 ) with strong bactericidal properties ( 2 - + H 2 O 2 OH - + OH - + O 2 ) and the Fenton reaction (H 2 O 2 + Fe 2+ Fe 3+ + OH - + OH - ) with the participation of pro-oxidant ions of transition metals (mostly Fe 2+ /Cu 2+ ) (51)

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